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KMID : 0364519920030030045
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Dong-A Journal Medicine
1992 Volume.3 No. 3 p.45 ~ p.55
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Rapid Diagnosis of Tuberculous Meningitis by Polymerase Chain Reaction
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Abstract
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Polymerase chain reaction(PCR) method is designed for in vitro enzymatic amplification of a specific segment of DNA. In the present work, PCR method was used to detect any M. tuberculosis DNA in the cerebrospinal fluid specimens from 14 patients
clinically diagnosed as tuberculous meningitis. We synthesized two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodation antigen of M. tuberculosis. The amplified 165bp genomic DNA of M. tuberculosis was
detected
in 3 patients (21.4%) with polyacrylamide gelelectrophoresis. Subsequent southern blot analysis confirmed these mycobacterial DNAs and detected another amplified DNA (28.6%) that was not seen on the polyacrylamide gels. Conventional detection
methods
including smear and culture for M. tuberculosis showed these specimens to be negative. It is recommended that PCR and combined southern blot analysis are most effective in early and rapid diagnosis of tuberculous meningitis.
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KEYWORD
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